Limited pepsin digestion of human plasma albumin.

Limited pepsin digestion of human plasma albumin.

Limited pepsin digestion of human plasma albumin at pH 3.5 and 0 degrees in the presence of octanoate caused cleavage at residue 307 of the albumin molecule to yield two fragments. Thw two fragments corresponding to the NH2- and the COOH-terminal halves of the molecule were isolated in yields of about 15%. The COOH-terminal fragment is a mixture in which about 85% of the molecules had an additi...

Relationship between the conformation of bovine serum albumin and its digestion by pepsin.

Pepsin Digestion of Rabbit and Sheep Antibodies

7S sheep antibody is similar to 7S rabbit antibody, insofar as it too can be digested with pepsin to yield a 5S fragment still capable of precipitating homologous antigen. The 5S fragment from rabbit as well as sheep antibody is capable of fixing guinea pig complement. However, this fixation differs from the fixation by 7S antibody, since preformed antibody-antigen aggregates containing either ...

Hydrogen Ion Concentration and Pepsin Digestion

One of the most striking peculiarities of enzyme action is the fact that the activity of the enzyme is limited to a definite range of acidity. If the solution is more or less acid than this the enzyme is practically inactive. SSrensen 1 showed that for a number of enzymes the determiuing factor was the hydrogen ion concentration and not the total acidity of the solution. In attempting to accoun...

Interaction of egg albumin and pepsin.

The interaction between enzymes and their substrates to form intermediate complexes has long been of interest. We have found that when pepsin and egg albumin are mixed in solution at about pH 4 a precipitate forms. The influence of protein and of ion concentrations on the amount of precipitate so formed has been studied. It has been found that the precipitate dissolves at higher ionic concentra...